Kukovetz, Kerri ; Hertel, Brigitte ; Schvarcz, Christopher R. ; Saponaro, Andrea ; Manthey, Mirja ; Burk, Ulrike ; Greiner, Timo ; Steward, Grieg F. ; Etten, James L. van ; Moroni, Anna ; Thiel, Gerhard ; Rauh, Oliver (2020)
A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae.
In: Viruses, 12 (10)
doi: 10.3390/v12101107
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Potassium ion (K) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K channels among these alga-infecting viruses has thus far been restricted to members of the family which are large, double-stranded DNA viruses within the phylum . Recent sequencing projects revealed that alga-infecting members of , another family within this phylum, may also contain genes encoding K channels. Here we examine the structural features and the functional properties of putative K channels from four cultivated members of . While all four proteins contain variations of the conserved selectivity filter sequence of K channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K over Na and a sensitivity to Ba. Thus, virus-encoded K channels are not limited to but also occur in the members of . The large sequence diversity among the viral K channels implies multiple events of lateral gene transfer.
Typ des Eintrags: | Artikel |
---|---|
Erschienen: | 2020 |
Autor(en): | Kukovetz, Kerri ; Hertel, Brigitte ; Schvarcz, Christopher R. ; Saponaro, Andrea ; Manthey, Mirja ; Burk, Ulrike ; Greiner, Timo ; Steward, Grieg F. ; Etten, James L. van ; Moroni, Anna ; Thiel, Gerhard ; Rauh, Oliver |
Art des Eintrags: | Bibliographie |
Titel: | A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. |
Sprache: | Englisch |
Publikationsjahr: | 29 September 2020 |
Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Viruses |
Jahrgang/Volume einer Zeitschrift: | 12 |
(Heft-)Nummer: | 10 |
DOI: | 10.3390/v12101107 |
Kurzbeschreibung (Abstract): | Potassium ion (K) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K channels among these alga-infecting viruses has thus far been restricted to members of the family which are large, double-stranded DNA viruses within the phylum . Recent sequencing projects revealed that alga-infecting members of , another family within this phylum, may also contain genes encoding K channels. Here we examine the structural features and the functional properties of putative K channels from four cultivated members of . While all four proteins contain variations of the conserved selectivity filter sequence of K channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K over Na and a sensitivity to Ba. Thus, virus-encoded K channels are not limited to but also occur in the members of . The large sequence diversity among the viral K channels implies multiple events of lateral gene transfer. |
ID-Nummer: | pmid:33003637 |
Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
Hinterlegungsdatum: | 12 Okt 2020 09:51 |
Letzte Änderung: | 20 Aug 2021 09:43 |
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