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A small viral potassium ion channel with an inherent inward rectification.

Eckert, Denise ; Schulze, Tobias ; Stahl, Julian ; Rauh, Oliver ; Etten, James L. van ; Hertel, Brigitte ; Schroeder, Indra ; Moroni, Anna ; Thiel, Gerhard (2019)
A small viral potassium ion channel with an inherent inward rectification.
In: Channels (Austin, Tex.), 13 (1)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Some algal viruses have coding sequences for proteins with structural and functional characteristics of pore modules of complex K channels. Here we exploit the structural diversity among these channel orthologs to discover new basic principles of structure/function correlates in K channels. The analysis of three similar K channels with ≤ 86 amino acids (AA) shows that one channel (Kmpv) generates an ohmic conductance in HEK293 cells while the other two (Kmpv, Kmpv) exhibit typical features of canonical Kir channels. Like Kir channels, the rectification of the viral channels is a function of the K driving force. Reconstitution of Kmpv and Kmpv in planar lipid bilayers showed rapid channel fluctuations only at voltages negative of the K reversal voltage. This rectification was maintained in KCl buffer with 1 mM EDTA, which excludes blocking cations as the source of rectification. This means that rectification of the viral channels must be an inherent property of the channel. The structural basis for rectification was investigated by a chimera between rectifying and non-rectifying channels as well as point mutations making the rectifier similar to the ohmic conducting channel. The results of these experiments exclude the pore with pore helix and selectivity filter as playing a role in rectification. The insensitivity of the rectifier to point mutations suggests that tertiary or quaternary structural interactions between the transmembrane domains are responsible for this type of gating.

Typ des Eintrags: Artikel
Erschienen: 2019
Autor(en): Eckert, Denise ; Schulze, Tobias ; Stahl, Julian ; Rauh, Oliver ; Etten, James L. van ; Hertel, Brigitte ; Schroeder, Indra ; Moroni, Anna ; Thiel, Gerhard
Art des Eintrags: Bibliographie
Titel: A small viral potassium ion channel with an inherent inward rectification.
Sprache: Englisch
Publikationsjahr: Dezember 2019
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Channels (Austin, Tex.)
Jahrgang/Volume einer Zeitschrift: 13
(Heft-)Nummer: 1
Kurzbeschreibung (Abstract):

Some algal viruses have coding sequences for proteins with structural and functional characteristics of pore modules of complex K channels. Here we exploit the structural diversity among these channel orthologs to discover new basic principles of structure/function correlates in K channels. The analysis of three similar K channels with ≤ 86 amino acids (AA) shows that one channel (Kmpv) generates an ohmic conductance in HEK293 cells while the other two (Kmpv, Kmpv) exhibit typical features of canonical Kir channels. Like Kir channels, the rectification of the viral channels is a function of the K driving force. Reconstitution of Kmpv and Kmpv in planar lipid bilayers showed rapid channel fluctuations only at voltages negative of the K reversal voltage. This rectification was maintained in KCl buffer with 1 mM EDTA, which excludes blocking cations as the source of rectification. This means that rectification of the viral channels must be an inherent property of the channel. The structural basis for rectification was investigated by a chimera between rectifying and non-rectifying channels as well as point mutations making the rectifier similar to the ohmic conducting channel. The results of these experiments exclude the pore with pore helix and selectivity filter as playing a role in rectification. The insensitivity of the rectifier to point mutations suggests that tertiary or quaternary structural interactions between the transmembrane domains are responsible for this type of gating.

ID-Nummer: pmid:31010373
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen)
Hinterlegungsdatum: 29 Apr 2019 10:44
Letzte Änderung: 20 Aug 2021 09:43
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