Boussebayle, Adrien ; Groher, Florian ; Suess, Beatrix (2019)
RNA-based Capture-SELEX for the selection of small molecule-binding aptamers.
In: Methods (San Diego, Calif.), 161
doi: 10.1016/j.ymeth.2019.04.004
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Despite their wide applicability, the selection of small molecule-binding RNA aptamers with both high affinity binding and specificity is still challenging. Aptamers that excel at both binding and structure switching are particularly rare and difficult to find. Here, we present the protocol of a Capture-SELEX that specifically allows the in vitro selection of small-molecule binding aptamers, which are essential building blocks for the design process of synthetic riboswitches and biosensors. Moreover, we provide a comparative overview of our proposed methodology versus alternative in vitro selection protocols with a special focus on the design of the pool. Finally, we have included detailed notes to point out useful tips and pitfalls for future application.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Boussebayle, Adrien ; Groher, Florian ; Suess, Beatrix |
| Art des Eintrags: | Bibliographie |
| Titel: | RNA-based Capture-SELEX for the selection of small molecule-binding aptamers. |
| Sprache: | Englisch |
| Publikationsjahr: | 15 Mai 2019 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Methods (San Diego, Calif.) |
| Jahrgang/Volume einer Zeitschrift: | 161 |
| DOI: | 10.1016/j.ymeth.2019.04.004 |
| Kurzbeschreibung (Abstract): | Despite their wide applicability, the selection of small molecule-binding RNA aptamers with both high affinity binding and specificity is still challenging. Aptamers that excel at both binding and structure switching are particularly rare and difficult to find. Here, we present the protocol of a Capture-SELEX that specifically allows the in vitro selection of small-molecule binding aptamers, which are essential building blocks for the design process of synthetic riboswitches and biosensors. Moreover, we provide a comparative overview of our proposed methodology versus alternative in vitro selection protocols with a special focus on the design of the pool. Finally, we have included detailed notes to point out useful tips and pitfalls for future application. |
| ID-Nummer: | pmid:30953759 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") |
| Hinterlegungsdatum: | 15 Apr 2019 09:47 |
| Letzte Änderung: | 25 Jul 2019 06:20 |
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