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Aquaporin PIP2;1 affects water transport and root growth in rice (Oryza sativa L.).

Ding, Lei and Uehlein, Norbert and Kaldenhoff, Ralf and Guo, Shiwei and Zhu, Yiyong and Kai, Lei (2019):
Aquaporin PIP2;1 affects water transport and root growth in rice (Oryza sativa L.).
In: Plant physiology and biochemistry : PPB, pp. 152-160, 139, ISSN 1873-2690, [Article]

Abstract

Aquaporins are key proteins in regulating water transport, plant growth and development. In this study, we investigated the function of plasma membrane intrinsic proteins (PIPs) in both yeast (Saccharomyces cerevisiae) and rice (Oryza sativa cv. Nipponbare). Three OsPIP1s (OsPIP1;1, OsPIP1;2 and OsPIP1;3) and four OsPIP2s (OsPIP2;1, OsPIP2;3, OsPIP2;4 and OsPIP2;5) were successfully amplified and expressed in yeast. Overexpression of OsPIP2s, especially OsPIP2;1, increased yeast membrane water permeability (P). Root hydraulic conductivity (Lpr) was decreased by approximately four-fold in OsPIP2; 1 RNAi knock-down plants, resulting in a decrease in OsPIP2;1 expression levels of 70% and 50% in line 3 and line 4, respectively, compared to the wild type (WT) plants. No significant differences in the photosynthetic rate, transpiration rate, mesophyll conductance and chloroplast CO concentration were observed between WT and OsPIP2; 1 RNAi plants. Higher stomatal conductance and intercellular CO concentrations were observed in line 3 plants than in WT plants. In addition, lower root total length, surface area, root volume and fewer root tips were found in the RNAi plants than in the WT plants. Finally, the RNAi plants were more sensitive to drought stress. The results indicate that PIP2; 1 plays an important role in the regulation of water transport and plant growth.

Item Type: Article
Erschienen: 2019
Creators: Ding, Lei and Uehlein, Norbert and Kaldenhoff, Ralf and Guo, Shiwei and Zhu, Yiyong and Kai, Lei
Title: Aquaporin PIP2;1 affects water transport and root growth in rice (Oryza sativa L.).
Language: English
Abstract:

Aquaporins are key proteins in regulating water transport, plant growth and development. In this study, we investigated the function of plasma membrane intrinsic proteins (PIPs) in both yeast (Saccharomyces cerevisiae) and rice (Oryza sativa cv. Nipponbare). Three OsPIP1s (OsPIP1;1, OsPIP1;2 and OsPIP1;3) and four OsPIP2s (OsPIP2;1, OsPIP2;3, OsPIP2;4 and OsPIP2;5) were successfully amplified and expressed in yeast. Overexpression of OsPIP2s, especially OsPIP2;1, increased yeast membrane water permeability (P). Root hydraulic conductivity (Lpr) was decreased by approximately four-fold in OsPIP2; 1 RNAi knock-down plants, resulting in a decrease in OsPIP2;1 expression levels of 70% and 50% in line 3 and line 4, respectively, compared to the wild type (WT) plants. No significant differences in the photosynthetic rate, transpiration rate, mesophyll conductance and chloroplast CO concentration were observed between WT and OsPIP2; 1 RNAi plants. Higher stomatal conductance and intercellular CO concentrations were observed in line 3 plants than in WT plants. In addition, lower root total length, surface area, root volume and fewer root tips were found in the RNAi plants than in the WT plants. Finally, the RNAi plants were more sensitive to drought stress. The results indicate that PIP2; 1 plays an important role in the regulation of water transport and plant growth.

Journal or Publication Title: Plant physiology and biochemistry : PPB
Volume: 139
Divisions: 10 Department of Biology
10 Department of Biology > Applied Plant Sciences
Date Deposited: 25 Mar 2019 10:48
Identification Number: pmid:30889480
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