TU Darmstadt / ULB / TUbiblio

Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1).

Dyall-Smith, Mike and Pfeifer, Felicitas and Witte, Angela and Oesterhelt, Dieter and Pfeiffer, Friedhelm (2018):
Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1).
In: Genes, 9 (10), pp. E493. ISSN 2073-4425,
[Article]

Abstract

The halophilic myohalovirus (ΦH) was first described in 1982 and was isolated from a spontaneously lysed culture of strain R1. Until 1994, it was used extensively as a model to study the molecular genetics of haloarchaea, but only parts of the viral genome were sequenced during this period. Using Sanger sequencing combined with high-coverage Illumina sequencing, the full genome sequence of the major variant (phiH1) of this halovirus has been determined. The dsDNA genome is 58,072 bp in length and carries 97 protein-coding genes. We have integrated this information with the previously described transcription mapping data. PhiH could be classified into Myoviridae Type1, Cluster 4 based on capsid assembly and structural proteins (VIRFAM). The closest relative was virus phiCh1 (φCh1), which shared 63% nucleotide identity and displayed a high level of gene synteny. This close relationship was supported by phylogenetic tree reconstructions. The complete sequence of this historically important virus will allow its inclusion in studies of comparative genomics and virus diversity.

Item Type: Article
Erschienen: 2018
Creators: Dyall-Smith, Mike and Pfeifer, Felicitas and Witte, Angela and Oesterhelt, Dieter and Pfeiffer, Friedhelm
Title: Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1).
Language: English
Abstract:

The halophilic myohalovirus (ΦH) was first described in 1982 and was isolated from a spontaneously lysed culture of strain R1. Until 1994, it was used extensively as a model to study the molecular genetics of haloarchaea, but only parts of the viral genome were sequenced during this period. Using Sanger sequencing combined with high-coverage Illumina sequencing, the full genome sequence of the major variant (phiH1) of this halovirus has been determined. The dsDNA genome is 58,072 bp in length and carries 97 protein-coding genes. We have integrated this information with the previously described transcription mapping data. PhiH could be classified into Myoviridae Type1, Cluster 4 based on capsid assembly and structural proteins (VIRFAM). The closest relative was virus phiCh1 (φCh1), which shared 63% nucleotide identity and displayed a high level of gene synteny. This close relationship was supported by phylogenetic tree reconstructions. The complete sequence of this historically important virus will allow its inclusion in studies of comparative genomics and virus diversity.

Journal or Publication Title: Genes
Journal volume: 9
Number: 10
Divisions: 10 Department of Biology
10 Department of Biology > Microbiology and Archaea
Date Deposited: 22 Oct 2018 10:56
Identification Number: pmid:30322017
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details