Hetzke, Thilo ; Vogel, Marc ; Gophane, Dnyaneshwar B. ; Weigand, Julia E. ; Suess, Beatrix ; Sigurdsson, Snorri Th. ; Prisner, Thomas F. (2019)
Influence of Mg2+ on the conformational flexibility of a tetracycline aptamer.
In: RNA (New York, N.Y.), 25 (1)
doi: 10.1261/rna.068684.118
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The tetracycline-binding RNA aptamer (TC-aptamer) is a synthetic riboswitch that binds the antibiotic tetracycline (TC) with exceptionally high affinity. Although a crystal structure exists of the TC-bound state, little is known about the conformational dynamics and changes upon ligand binding. In this study, pulsed electron paramagnetic resonance techniques for measuring distances (PELDOR) in combination with rigid nitroxide spin labels (Çm spin label), was used to investigate the conformational flexibility of the TC aptamer in the presence and absence of TC at different Mg concentrations. TC was found to be the essential factor for stabilizing the tertiary structure at intermediate Mg concentrations. At higher Mg concentrations, Mg alone is sufficient to stabilize the tertiary structure. In addition, the orientation of the two spin-labeled RNA helices with respect to each other was analyzed with orientation-selective PELDOR and compared to the crystal structure. These results demonstrate for the first time the unique value of the Çm spin label in combination with PELDOR to provide information about conformational flexibilities and orientations of secondary structure elements of biologically relevant RNAs.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Hetzke, Thilo ; Vogel, Marc ; Gophane, Dnyaneshwar B. ; Weigand, Julia E. ; Suess, Beatrix ; Sigurdsson, Snorri Th. ; Prisner, Thomas F. |
| Art des Eintrags: | Bibliographie |
| Titel: | Influence of Mg2+ on the conformational flexibility of a tetracycline aptamer. |
| Sprache: | Englisch |
| Publikationsjahr: | Januar 2019 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | RNA (New York, N.Y.) |
| Jahrgang/Volume einer Zeitschrift: | 25 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1261/rna.068684.118 |
| Kurzbeschreibung (Abstract): | The tetracycline-binding RNA aptamer (TC-aptamer) is a synthetic riboswitch that binds the antibiotic tetracycline (TC) with exceptionally high affinity. Although a crystal structure exists of the TC-bound state, little is known about the conformational dynamics and changes upon ligand binding. In this study, pulsed electron paramagnetic resonance techniques for measuring distances (PELDOR) in combination with rigid nitroxide spin labels (Çm spin label), was used to investigate the conformational flexibility of the TC aptamer in the presence and absence of TC at different Mg concentrations. TC was found to be the essential factor for stabilizing the tertiary structure at intermediate Mg concentrations. At higher Mg concentrations, Mg alone is sufficient to stabilize the tertiary structure. In addition, the orientation of the two spin-labeled RNA helices with respect to each other was analyzed with orientation-selective PELDOR and compared to the crystal structure. These results demonstrate for the first time the unique value of the Çm spin label in combination with PELDOR to provide information about conformational flexibilities and orientations of secondary structure elements of biologically relevant RNAs. |
| ID-Nummer: | pmid:30337459 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 22 Okt 2018 10:51 |
| Letzte Änderung: | 06 Nov 2020 07:09 |
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