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The iNAPO Project: Biomimetic Nanopores for a New Generation of Lab-on-Chip Micro Sensors

Ensinger, Wolfgang and Ali, Mubarak and Nasir, Saima and Duznovic, Ivana and Trautmann, Christina and Toimil-Molares, Maria Eugenia and Distefano, Giuseppa R. and Laube, Bodo and Bernhard, Max and Mikosch-Wersching, Melanie and Schlaak, Helmut F. and El Khoury, Mario (2018):
The iNAPO Project: Biomimetic Nanopores for a New Generation of Lab-on-Chip Micro Sensors.
In: International Journal of Theoretical and Applied Nanotechnology, Avestia Publishing, pp. 21-28, 6, ISSN 19291248,
DOI: 10.11159/ijtan.2018.004,
[Online-Edition: https://doi.org/10.11159/ijtan.2018.004],
[Article]

Abstract

In nature, ion conducting nanopores play a vital role for the function of living cells. They undergo gating processes where they open and close upon an external stimulus, such as the presence of a particular biomolecule, the ligand. When the gating process is observed and is quantitatively measured, one can derive data about the presence and the amount of the ligand. Hence, the nanopores can be utilized for specific sensing. However, biological nanopores are embedded in a biological cell membrane that is fragile and unstable with respect to storage and application. The iNAPO (ion conducting nanopores) project aims at combining robust polymer-based nanopores with protein-based biological nanopores, thus combining the selectivity and sensitivity of the latter with the stability and processibility of the first ones. This paper describes the different steps in the fabrication of ion conducting nanopores. It begins with ion irradiation of polymer foils, combined with chemical etching of the ion damage tracks into nanopores. By means of chemical coupling reactions, the nanopore walls are functionalized with particular molecules which react or bioconjugate with the molecules to be analyzed. As an example, a recent result on sensing a physiologically active phosphorus-based anion is shown. By means of a complexation reaction with Zn-di(picolyl)amine, the selective measurement of the concentration of the anion pyrophosphate is demonstrated. In the final step of the project, the nanopores will be incorporated into a Lab-on-Chip system for applications in e.g. medical diagnostics and environmental analysis.

Item Type: Article
Erschienen: 2018
Creators: Ensinger, Wolfgang and Ali, Mubarak and Nasir, Saima and Duznovic, Ivana and Trautmann, Christina and Toimil-Molares, Maria Eugenia and Distefano, Giuseppa R. and Laube, Bodo and Bernhard, Max and Mikosch-Wersching, Melanie and Schlaak, Helmut F. and El Khoury, Mario
Title: The iNAPO Project: Biomimetic Nanopores for a New Generation of Lab-on-Chip Micro Sensors
Language: English
Abstract:

In nature, ion conducting nanopores play a vital role for the function of living cells. They undergo gating processes where they open and close upon an external stimulus, such as the presence of a particular biomolecule, the ligand. When the gating process is observed and is quantitatively measured, one can derive data about the presence and the amount of the ligand. Hence, the nanopores can be utilized for specific sensing. However, biological nanopores are embedded in a biological cell membrane that is fragile and unstable with respect to storage and application. The iNAPO (ion conducting nanopores) project aims at combining robust polymer-based nanopores with protein-based biological nanopores, thus combining the selectivity and sensitivity of the latter with the stability and processibility of the first ones. This paper describes the different steps in the fabrication of ion conducting nanopores. It begins with ion irradiation of polymer foils, combined with chemical etching of the ion damage tracks into nanopores. By means of chemical coupling reactions, the nanopore walls are functionalized with particular molecules which react or bioconjugate with the molecules to be analyzed. As an example, a recent result on sensing a physiologically active phosphorus-based anion is shown. By means of a complexation reaction with Zn-di(picolyl)amine, the selective measurement of the concentration of the anion pyrophosphate is demonstrated. In the final step of the project, the nanopores will be incorporated into a Lab-on-Chip system for applications in e.g. medical diagnostics and environmental analysis.

Journal or Publication Title: International Journal of Theoretical and Applied Nanotechnology
Volume: 6
Publisher: Avestia Publishing
Uncontrolled Keywords: Ion Track Etching, Polymer Membrane, Nanopores, Biomimetic Sensor, Nanosensor, CurrentPotential Measurements.
Divisions: 11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Material Analytics
11 Department of Materials and Earth Sciences > Material Science > Ion-Beam-Modified Materials
Date Deposited: 06 Jul 2018 11:54
DOI: 10.11159/ijtan.2018.004
Official URL: https://doi.org/10.11159/ijtan.2018.004
Funders: This work has been supported in the frame of the LOEWE project iNAPO by the Hessen State Ministry of Higher Education, Research and the Arts.
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